Posted 1/11/2024, 3:58:12 PM
New machine learning approach better predicts CRISPRi guide efficiency using multiple data sources
- Researchers developed a machine learning approach to better predict the efficacy of CRISPRi gene silencing
- The approach integrates data from multiple genome-wide CRISPRi screens
- It outperforms existing methods in predicting guide RNA efficiency
- Gene expression characteristics have a bigger impact than the guide RNA itself
- The approach will help plan more effective CRISPRi experiments in future